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This ensures the matrix is properly cleared before new collagen and elastin are laid down

Using the biweight midcorrelation, a similarity matrix was computed from which a signed network was constructed using a soft-thresholding power of 14 (MBH, DVC) or 16 (LPBN), maximising the scale-free topology R2 fit while keeping mean connectivity to a value 100

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On the fourth day, 1 mg ml of l-(4, 5-Dimethylthiazol-2-yl) 3, 5-diphenyIformazan reagent (MTT, Sigma- Aldrich) prepared in PBS was added to the cells and incubated for 4 h

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